Scientific communications

How to fully exploit the mixed leukocyte reaction assay?
for assessing immunotherapeutics on dendritic cell function and mediated immune response
While immunological synapse that occurs between T lymphocytes and dendritic cells (DCs) is a key component for an effective immune response, DC subpopulations are generated involving many signaling cues whose the nature only determines their functional properties

Ascites is a pathological peritoneal fluid accumulation most frequently associated with ovarian cancer, among few others, thereby creating a quite unique tumor microenvironment in which cancer cells spread.

As Olink®-certified service Provider, Explicyte offers now high proteomic analysis capabilities for comprehensive protein biomarker discovery using Olink® Target technology.

Olink®’s Target96 biomarker panels are dedicated to many diseases and/or biological processes. You can directly download them from the form below and do not hesitate to let us know your proteomics projects!

Development and characterization of a novel anti-PD1 resistant sarcoma mouse model

Soft Tissue Sarcoma (STS) is known to be refractory to current cancer immunotherapies including the PD1 immune checkpoint inhibitor (ICI) (Toulmonde et al., 2018), thus claiming the development of novel therapeutic strategies aiming at improving the current clinical benefit.

Drawing on our expertise in multiplexed-immunohistofluoresence (IHF) and immunohistochemistry (IHC) assays, we have already developed and validated a series of markers & panels to assess several markers of interest as well as the abundance and spatial distribution of lymphoid and myeloid immune cell subsets. While bringing key answers to address specific questions, our capacities have recently been highlighted in new scientific publications of collaborative works with Institut Bergonié, which we are delighted to share thereafter.

While one of the promising cancer immunotherapy challenges is the potentiation of immune cell-induced tumor cell attacks, in vitro immune cell-mediated killing is recognized among the most relevant functional measures to evaluate the ability of candidate drugs to promote the functions of effector immune cells. We set up and designed specialized in vitro immune cell killing assays - either T cell- or NK-mediated - to test candidate compounds or antibodies for their immunomodulatory properties in enhancing the immune response against tumor cells.

To pursue our commitment to expand cutting-edge approaches for the identification and discovery of novel biomarkers and actionnable therapeutic targets, we implemented the GeoMx DSP technology platform which was already referenced in scientific publications this year, notably in JITC and Nature Medicine.

The development of immune checkpoint blockers (ICB) has considerably changed the therapeutic armamentarium for the management of cancer. Blocking the PD-1/PD-L1 axis interaction has demonstrated remarkable anti-cancer activity and has led to approval of anti-PD-1/PD-L1 drugs in several solid tumors. However, most patients receiving PD(L)-1 blockers do not derive clinical benefit. Therefore, there is a crucial need to decipher mechanisms underlying sensitivity / resistance to cancer immunotherapies which can ultimately lead to the identification of novel therapeutic targets.

Take advantage of our upcoming shuttle session - scheduled early-December - to cost-effectively run your candidates on our robust DC differentiation and Mixed Leukocyte Reaction (MLR) assays thereby saving up to 25% on initial cost. 

Characterized through adenosine-mediated suppression results, our validated DC-based assays represent valuable tools for assessing adenosine pathway modulators as well as innovative candidate immunotherapeutics for their potential to enhance DC-mediated T cell stimulation. 

Differentiation and maturation of competent dendritic cells (DCs) is critically dependent on their microenvironment. Among factors which can, in cancer, reach high levels in the tumor microenvironment, adenosine - one of the major immunosuppressive pathways - is known to modulate the function of many cell subsets, including DCs. Adenosine receptor stimulation skews DC differentiation toward a distinct cell population which has, unlike normal myeloid DCs, tolerogenic and suppressive phenotype expressing high levels of proinflammatory and immune suppressor factors, as well as impaired allostimulatory capacity.