Cell migration & chemotaxis evaluation

Cell migration is a key critical process of live cells involved in many biological processes – physiological and pathological - including normal development, immune response, and many pathological processes e.g. cancer metastasis, tumor immune infiltration, inflammation, wound healing…
The study of cell migration in cancer research is of particular interest as two of the main challenges in the development of novel therapeutic strategies are i) the inhibition of metastatic progression of cancer cells and ii) stimulation of the recruitment of immune cells and their infiltration into the tumor masses. Indeed, while for cancer spread throughout the body cancer cells must migrate and invade through extracellular matrix, and intravasate/extravasate, chemotactic immune cell recruitment and infiltration into the tumor (e.g. effector T cells, regulatory T cells (Treg), Th17, macrophages, dendritic cells) is primordial for effective anti-tumor immune response to counter disease progression.
 
In this respect, Explicyte is offering valuable in vitro cell-based 96-w plate assays to evaluate compounds effects (with potential chemoattractive or chemorepulsive properties) on the migration of immune or cancer cells, respectively. Based on live cell time-lapse imaging, our assays unlike conventional single time-point chemotaxis/migration assays allow for full time course chemotaxis/cell migration profiles.
 
 

Assay principle


  • Cells are seeded in the upper chamber onto a 96-w format translucent porous membrane (coated, if needed).
  • Insert is placed in the lower chamber and immersed in the migration medium
  • Real-time visualization & kinetic monitoring of chemotaxis/cell migration by live cell imaging
  • Whole-well images of cells on the bottom and top of the porous membrane are acquired and processed for data analysis & quantification. Directed cell migration is analyzed as cell area on the bottom or top side of the membrane for adherent and non-adherent cells, respectively, that migrate down the pores.
 

Illustrative data

Chemotactic cancer cell migration
Chemotactic FBS-induced migration of fibrosarcoma HT-1080 cancer cells. HT-1080 cells are seeded in a low serum-containing medium and monitored for migration toward serum gradient. Total phase cell area on the bottom side of the membrane is measured and normalized to the initial value, and plotted over a 96h time course. Lower chamber serum dose-dependently induces directed cell migration from the top to the bottom side of the membrane.    

Chemotactic FBS-induced migration of fibrosarcoma HT-1080 cancer cells. HT-1080 cells are ...

Chemotactic T cell migration induction
Chemotactic FBS-induced migration of fibrosarcoma HT-1080 cancer cells. HT-1080 cells are seeded in a low serum-containing medium and monitored for migration toward serum gradient. Total phase cell area on the bottom side of the membrane is measured and normalized to the initial value, and plotted over a 96h time course. Lower chamber serum dose-dependently induces directed cell migration from the top to the bottom side of the membrane.    

Chemoattractive effect of SDF1 on T Jurkat cell migration. Chemotaxis monitoring and quantification ...

Chemotactic T cell migration inhibition
Chemotactic Jurkat T cell migration is induced by SDF1𝛂 and reversed in the presence of a CXCR4 antagonist. While SDF1𝛂 time- and dose-dependently promotes Jurkat T cell migration, this chemoattractive effect is antagonized by AMD-3465, a potent CXCR4 antagonist. 

Chemotactic Jurkat T cell migration is induced by SDF1𝛂 and reversed in the presence ...

Chemotactic Neutrophil migration
Neutrophil chemotaxis toward fMLP and IL-8. Chemotactic neutrophil migration is dose-dependently induced by fMLP (a) and IL-8 (b), rapidly over a 6h period. These chemoattractive effects are completely abolished in the presence of a PLCƔ inhibitor (U73122).   

Neutrophil chemotaxis toward fMLP and IL-8. Chemotactic neutrophil migration is dose-dependently ...