Immunohistochemistry & tissue-based analysis

In parallel with its flow cytometry-related know-how and platform, explicyte has extensive experience performing immunohistochemistry / immunohistofluorescence and related tissue analysis. Detection of immunohistochemical staining to assess specific markers characteristic either of cellular and molecular processes or to identify specific cell subsets as to profile an immune response to anti-cancer treatment, for instance, is an integral part of explicyte services that we are providing to build our clients’ studies and have an in-depth insight into the pattern characteristics and treatment responses. While we are continuously enriching our validated marker panels, our immunohistochemistry-related services are also widely proposed to study the expression, distribution and localization of intended biomarkers, in many types of tissues (tumors, xenografts, organs, normal tissue…) either frozen or paraffin-embedded, through either a standardized or customized work as to meet our specific clients’ needs.
Tumor infiltration in orthotopic glioma
Orthotopic glioma tumor-bearing model is characterized by tumor-infiltrating immune cells.
Hematoxylin-associated IHC staining of brain sections of GL261 glioma-bearing mice (top left images) shows the presence of F4/80+ macrophages and CD8+ cytotoxic lymphocytes infiltrating the tumor as depicted in a & b, respectively. Presence of PD1-expressing immune cells infiltrating the tumor  is highlighted in c.

Orthotopic glioma tumor-bearing model is characterized by tumor-infiltrating immune cells. ...

Tumor infiltration in colon tumor
Tumor-infiltrating lymphocyte cells characterizing the subcutaneous syngeneic CT26 colon tumor model. Hematoxylin-associated IHC staining of CT26 tumors shows the presence of  cytotoxic CD8+ (A) and CD4+ (B) lymphocytes

Tumor-infiltrating lymphocyte cells characterizing the subcutaneous syngeneic CT26 colon tumor ...