Flow cytometry for immune response profiling


In addition to its relevant flow cytometry platform, explicyte has extensive know-how and expertise for the multiplexed detection and analysis of markers of interest. Analyzing series of specific biomarkers or markers (intracellular, surface, or secreted) provides indeed a thorough insight into cellular function, thereby enabling the characterization of pathology-underlying processed or of mechanisms of action of drug candidates. Also, we provide flow cytometry-based services as gold standard for immunophenotyping and immune response profiling, for which both standardized and customized marker panels are performed, so as to meet our specific clients’ needs and support their drug discovery & screening programs as well as biomarker expression and pharmacokinetic assessment.
 

Key features and advantages of flow cytometry

  • The gold-standard for immunophenotyping and immune response profiling: marker analysis in specific cell subsets.
  • Well-suited for multiplexed detection of markers of interest (intracellular, surface markers, phosphoproteins) and compatible with high throughput.
  • Appropriate for multiplexed detection of secreted biomarker molecules e.g. cytokines in a multitude of biological samples such as supernatants, blood derivatives, and tissue homogenates).

Platform

MACSQuant 10 (Miltenyi)

  • 3-laser flow cytometer for multiplexed marker detection

Biological models

Cell cultures, ex vivo tumor samples, spleen and blood samples, blood derivatives and supernatants for e.g. multiplexed cytokine detection)

  • Many sample vessels, including 96-w & 384-w plates

 
In vitro PBMC immunophenotyping
aCD3-mediated PBMC stimulation induces CD4 and CD8 lymphocyte activation and decease in the proportion of dendritic cells

aCD3-mediated PBMC stimulation induces CD4 and CD8 lymphocyte activation and decease in the ...

Profiling of GL261 glioma tumor-infiltrating immune cells
Flow cytometry analysis shows infiltration by myeloid CD45+ CD11b+ cells. MDSCs CD11b+ are identified as granulocytic (Gr1High) or monocytic (Gr1Low) (left plot). Further analysis within CD11b+ cell populations (right plot) demonstrates the presence of myeloid Ly6G+ Ly6CInt and Ly6G+ Ly6CLow populations - known to harbor immunosuppressive properties and participate in the tumor immune escape.

Flow cytometry analysis shows infiltration by myeloid CD45+ CD11b+ cells. MDSCs CD11b+ are ...