Validated markers for histology

To deal with the current challenge of a qualitative and quantitative tumor immune landscape characterization, we have set up a fully integrated platform spanning from FFPE slide preparation and staining, to image digitization and analysis. This combined approach – taking advantage of a series of validated markers – can be applied in monoplex or multiplex mode through conventional immunohistochemistry (IHC) or immunohistofluorescence (IHF).


Cell line markers for immunohistochemistry (IHC)

Name

Species

Markers

ICI Markers Human CD8 / PDL1
TLS Human CD3 / CD20
TLS maturity Human CD3 / CD20 / CD23
Tumor_I Human CD163 / CD8 / CK7
Tumor_II Human CD163 / CD8 / CDX2



Cell line markers for immunohistofluorescence (IHF)

Name

Species

Markers

B cells_I Human CD3 / CD20 / CD27 / Col1A1 / IgG / Mum1
B cells_II Human CD3 / CD20 / CD23 / Col1A1 / IgG / Mum1
Fibroblast_I Human CD45 / CD31 / CD248 / Col1A1 / ISLR / PDGFRα
Fibroblast_II Human CD45 / CK7 / Col1A1 / PDL1 / TGFb1 / TGM2
Immuno_I Human CD3 / CD8 / Foxp3 / CD20 / PD1 / Granzyme B / Ki67
Immuno_II Human CD3 / CD56 / 4-1BB / TIGIT / PVR
Immuno_III Human CD4 / CD8 / CD56 / Granzyme A / Foxp3
Metabolic_I Human CD45 / CD8 / Col1A1 / DDR1 / SLC7A11 / SLC3A2
Myeloid_Exhaustion Human CD33 / CD66b / CD68 / CD163 / PD1 / PDL1 / TIM3 / VISTA
Myeloid_I Human CD1c / CD11c / CD68 / CD83 / HLA-DR
Myeloid_II Human CD11b / CD15 / CD66b / CD163 / Arg1 / MPO
Myeloid_III Human CD11b / CD11c / CD14 / CD45 / CD68 / cMAF / Elastase / MPO
T cells_I Human CD45 / CD4 / CD8 / Foxp3 / Ki67 / PanCK
T cells_II Human CD4 / CD8 / CD20 / CK7 / Foxp3 / ICOS / TIGIT
T_Exhaustion_I Human CD3 / CTLA4 / PD1 / TIM3 / LAG3 / TIGIT
T_Exhaustion_II Human CD8 / CD39 / CD45 / CK7 / LAG3 / PD1 / TIGIT / TIM3
T_NK_Exhaustion Human CD3 / CD56 / LAG3 / NKG2A / PD1 / TCRγδ / TIGIT / TIM3
Tfh Human CD4 / CD8 / CD20 / CXCR5 / cMAF / PD1
Tfh_Tfr Human CD4 / CD8 / CD45 / CD56 / CXCR5 / Foxp3 / ICOS / PD1
TLS activity Human CD4 / CD8 / CD20 / Ki67 / Foxp3
TLS maturity Human CD4 / CD8 / CD20 / CD21 / CD23
Tumor_I Human CD8 / CD163 / PD1 / PDL1 / IDO1 / PanCK
Tumor_II Human CD8 / CD163 / PD1 / PDL1 / IDO1 / CK19
Tumor_III Human CD8 / CD163 / CD68 / cMAF / pSTAT1 / CK20
Tumor_IV Human CD8 / PDL1 / IDO1 / PanCK
Angiogenesis Mouse HIF1α / CD31 / NG2
Immuno_I Mouse CD45 / CD8 / CD11b / Arg1 / PD1 / PDL1
Immuno_II Mouse CD45 / CD8 / Vimentin / F4/80 / PDL1 / PD1
Immuno_III Mouse CD45 / CD3 / Vimentin / F4/80 / PDL1 / PD1
Immuno_IV Mouse CD45 / CD4 / CD8 / Vimentin / PDL1 / PD1
Immuno_V Mouse CD45 / CD4 / CD8 / CD11b / Elastase / Foxp3
T cells Mouse CD45 / CD3 / CD4 / CD8 / Foxp3 / Vimentin



Download our validated marker list