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Figure 1. Evaluation of the expression of CD16 and CD56 in primary NK cells by flow cytometry, after NK isolation from PBMCs (A) and following 24h activation (B).
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Figure 3. Combination of NK cells with anti-HER2 or anti-EGFR antibodies enhances NK-mediated cytotoxicity in SKOV3 ovarian tumor cells, in a NK ratio-dependent manner. SKOV3 tumor cells were cultured under control, Cetuximab-, or Trastuzumab-treated conditions and IL2- activated primary NK cells were added at different E:T ratios. 24h later, supernatants were collected and analyzed for IFN𝜸 release by HTRF as a surrogate of NK-induced cell toxicity. While IFN𝜸 levels are increased in a NK-ratio dependent manner, Cetuximab and Trastuzumab treatments of tumor cells promote this increase whatever the E:T ratio, thereby showing an ADCC activity.
Figure 4. Dose-dependent anti-EGFR antibody (Cetuximab)-dependent cell cytotoxicity in SKOV3 ovarian tumor cells. SKOV3 tumor cells were cultured under control or increasing doses Cetuximab-treated conditions and IL2- activated primary NK cells were added at an appropriate E:T ratio (3:1). (A) Integration of live cell imaging data at 15h after NK cell addition as AUC of tumor cell apoptosis as a surrogate measure of NK-mediated tumor cytotoxicity. (B) 24h following NK cell addition, supernatants were collected and analyzed for IFN𝜸 release
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