In Vitro Immune Cell Killing Of Tumor Cells

Cancer immunoediting is a dynamic process which consists in three phases including elimination, equilibrium and tumor immune escape. During the elimination step there is an engagement of effector cells such as natural killer or T cells which initiate tumor cell death – an event defective in case of tumor progression. Such key mechanism thus warrants investigation to optimize immune cell mediated killing of tumor cell. To this aim, we developed immune / tumor cells co-culture on a 96-well throughput platform to evaluate the capacity of candidate compounds to promote immune cell dependent tumor cell death alone and/or in combination with immune checkpoint inhibitors.

In Vitro Immune Cell Killing Of Tumor Cells
Immune cell killing of murine CT26 colorectal cancer cells is enhanced by CTLA4 blockade. Colorectal cancer cells (CT26) are cultivated in the presence of anti-CD3 activated splenocytes in the presence or absence of anti-CTLA4 Ab. Cells are followed by live-cell imaging for both proliferation and apoptosis (Caspase 3/7 – green objects).


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