Visium Whole-Transcriptome Panel
- Eligible samples: FFPE & frozen tissues (mouse & human)
- Panel: ~18K protein-coding genes, with optional immuno-oncology protein add-on
- Resolution: 55µm barcoded spots
- Capture area: Up to 11 mm x 11 mm
Advantage: FFPE-compatible, cost-efficient, large field of capture
Visium HD Whole-Transcriptome Panel
- Eligible samples: FFPE & frozen tissues (mouse & human)
- Panel: ~18K protein-coding genes
- Resolution: 2μm barcoded squares
- Capture area: 6.5 x 6.5 mm (2 capture areas per slide)
Advantage: FFPE-compatible, enhanced sensitivity & resolution
Visium HD 3’ Gene Expression
- Eligible samples: Fresh-frozen tissues (any species)
- Panel: 3’poly(A) whole-transcriptome capture
- Resolution: 2μm barcoded squares
- Capture area: 6.5 x 6.5 mm (2 capture areas per slide)
Advantage: Multi-species, ideal for de novo discovery (isoform, TCR/BCR, SNV detection)
Sample sourcing & in-house processing
- Custom sourcing access to human tumor specimens via accredited French biobanks
- Rigorous sample QC: RNA integrity assessed by RNAscope/DV20
Standard data package
- Raw data processed with 10x Space Ranger: alignment, spatial barcode deconvolution, gene-expression quantification per spot
- Matching of gene expression map with high-resolution image
- Web summary report, including key metrics, clustering, top features by cluster
Custom data science
- Customized analysis based on proprietary workflows & algorithms
- Custom nuclei and cell segmentation to assign transcripts to inferred cells
- Manual cluster annotation to reveal tissue architecture
- Custom pipeline and graphs: integration of multiple samples, differential expression, pathways and cell-cell communication analyses
Experts
in Immuno-Oncology
- Proven Expertise: +30 peer-reviewed publications in the field of immuno-oncology in the last 5 years
- 10-year experience: Over 10,000 samples processed to explore mechanisms associated with sensitivity to cancer therapies.
- Comprehensive Data Integration: Enhance your dataset with additional information from H&E staining and mIHF/IHC assays.
Personalized
approach
- Tailored experimental design – sample and panel selection – to align with your unique requirements and specific sample types.
- Dedicated Expertise: Benefit from a dedicated PhD study director who will tailor your experimental plan and offer thorough discussions of your outcomes.
- Comprehensive Bioinformatics Support: Utilize our in-house bioinformatics pipeline crafted to maximize the value of your spatial dataset.
Your contacts
Talk to our team !
Paul Marteau, PharmD (preclinical study director), Imane Nafia, PhD (CSO), Loïc Cerf, MSc (COO), Alban Bessede, PhD (founder, CEO), Jean-Philippe Guégan, PhD (CTO)
Tell us about your project !
Visium HD services for Whole-Transcriptome Spatial Biology
Explicyte provides spatial transcriptomics services based on the Visium Cytassist HD platform by 10x Genomics. Complementing our single-cell platforms (Xenium, Chromium), the Visium HD creates opportunities for faster and cost-effective spatial transcriptomics data. Our team can source human specimens to meet your need, and provide custom data science services to make the most of your dataset.