Applicable to a Wide Range of Samples
- Species: human or rodent specimens
- Formats: tumor biopsies, surgical pieces, peripheral blood, organoids, cell lines, …
- In-house sample processing: starting from fresh, frozen, or fixed (FFPE) samples
Comprehensive Single-Cell Sequencing
- Single-cell gene expression & immune profiling: using probe-based (Flex), 3’ or 5’ gene expression
- Multiplexing up to 128 samples per chip with Flex technology
- High-throughput with the 10X-powered Chromium X platform
- Compatible with multi-omics approaches cell surface proteins, TCR/BCR sequencing, antigen specificity
Basic & Tailored In-House Data Science
- Data mapping, cluster identification, and characterization (e.g., abundance)
- Differential gene expression – creation of heatmaps and volcano plots
- In-depth statistical analysis to decipher mechanisms of action at a cellular level, explore signaling pathways, gene signatures, and immune cell estimation (data deconvolution)
Custom Workflow from Sample to Data Analysis
- Collaborate with our experts to select the optimal sequencing protocol from a wide range of reagents to save time and money
- Sample Preparation: receive guidance on optimal sample collection and preparation for single-cell analyses
- Anticipate future needs to explore your sample further (ATAC-seq, BCR/TCR-seq)
Experts
in Immuno-Oncology
- Proven Expertise: 30+ peer-reviewed publications in the field of immuno-oncology over the past 5 years
- Extensive Experience: 10,000+ samples processed to explore mechanisms associated with sensitivity to cancer therapies
- Advanced Technology: In-house cutting-edge single-cell platforms to investigate the heterogeneity of your samples. Discover our platform for single-cell spatial transcriptomics
Personalized
approach
- Customized sequencing strategies line with your specific needs and the features of your samples.
- A dedicated study director (PhD) to tailor the experimental plan to your need and discuss results
- In-house bioinformatics pipeline to maximize the value and insights derived from your dataset
Differential gene expression in responding and non-responding NSCLC patients treated with immunotherapy
Formalin-fixed, paraffin-embedded (FFPE) blocks from immunotherapy responder and non-responder NSCLC patients (n=2 per group) were dissociated and processed using the 10X Genomics Flex Gene Expression kit. Single-cell RNA sequencing (scRNA-seq) was conducted on the Illumina NovaSeq 6000 platform, and data were visualized using Uniform Manifold Approximation and Projection (UMAP). A target gene differentially expressed between the two conditions was identified within the tumor cell cluster. The findings were further validated by immunohistochemistry (IHC) targeting the identified marker on the same FFPE blocks (40X objective).
Your contacts
Talk to our team !
Paul Marteau, PharmD (preclinical study director), Imane Nafia, PhD (CSO), Loïc Cerf, MSc (COO), Alban Bessede, PhD (founder, CEO), Jean-Philippe Guégan, PhD (CTO)
Tell us about your project !
Single-cell RNA sequencing for immuno-oncology I 10x Chromium-based CRO Services
Welcome to Explicyte’s premier Single Cell Analysis services, designed to propel advancements in immuno-oncology (IO). Utilizing cutting-edge technologies from industry leader 10x Genomics, we empower scientists to explore the intricate tumor-immune landscape with exceptional precision. Why Single Cell Analysis in Immuno-Oncology? Immuno-oncology leverages the body’s immune system to fight cancer, but the tumor microenvironment is highly heterogeneous, containing diverse cell populations with unique functions and states. Understanding this complexity is essential for identifying and characterizing rare immune cell subsets that play pivotal roles in tumor suppression or progression. It also enables the unraveling of intricate interactions between tumor and immune cells to pinpoint potential therapeutic targets, investigating mechanisms by which cells evade immune detection or develop resistance to therapies, and tailoring immunotherapies based on the specific cellular composition and dynamics of individual tumors.