Tumor killing assays
Tumor antigen-specific T-cell killing assays
Does your vaccine or tumor antigen candidate generate a functional tumor-specific CD8 T-cell response? Or can your compound enhance dendritic-cell antigen presentation and the priming of tumor-reactive T cells?
This assay connects antigen presentation and CD8 T-cell priming to antigen-specific tumor killing, enabling the evaluation of both antigen candidates and immune-modulating compounds within a defined antigen–tumor system.
Tumor antigen-specific T-cell killing: format and readouts
- Format
- Miniaturized DC cultures, DC–CD8 co-cultures, and CD8–tumor co-cultures.
- Model
- Human DC-primed CD8 T cells co-cultured with antigen-positive target tumor cells.
- Readouts
- Tumor-cell apoptosis and count by live-cell imaging, alongside IFN-γ release and other cytokines or immune readouts as relevant to the model.
- Reference
- A qualified Melan-A / SK-MEL-5 system is available as a positive reference model for antigen-specific CD8 T-cell priming and tumor-cell killing. For sponsor-defined antigens or vaccine candidates, study-specific controls are selected according to the antigenic format, HLA restriction and tumor model.
- On request
- Flow cytometry immunophenotyping, including DC differentiation and maturation markers, cytokine/secretome analysis, proteomics and transcriptomics.
Killing after antigen-priming
Killing after control priming
DC-mediated priming of CD8 T cells drives antigen-specific killing of SK-MEL-5 (Melan-A⁺) melanoma cells. SK-MEL-5 targets were co-cultured with CD8 T cells primed by either untreated or Melan-A-treated dendritic cells. Tumor-cell count and apoptosis were tracked over ~72 h as functional measures of immune-cell-mediated killing.
Imane Nafia, PhD
Chief Scientific Officer, Explicyte
Assay principle
How the tumor antigen-specific T-cell killing assay works
1
Antigen loading and dendritic-cell modulation
Dendritic cells are exposed to the antigen of interest, such as a peptide, vaccine construct or tumor-derived material. Alternatively, a defined antigen such as Melan-A can be used to evaluate compounds that enhance dendritic-cell maturation, antigen processing or presentation.
2
CD8 T-cell priming
3
Antigen-specific tumor-cell killing
Primed CD8 T cells are challenged with antigen-positive tumor cells. Tumor-cell apoptosis and proliferation are monitored by live-cell imaging, while cytokine release and optional immune profiling characterize the associated T-cell response.
Representative data
Antigen-specific tumor cell killing case study
Making a difference as a preclinical CRO in oncology
Why work with Explicyte
A decade of in vitro immuno-oncology
100+ in-vitro campaigns, 30+ peer-reviewed publications in immuno-oncology journals, across the mechanisms that antigen-specific killing depends on.
Built around your program
One study director (PhD level) from experimental design through final-report discussion, shaping the readouts and treatment windows around your mechanism of action.
Mechanism, not just a number
Add immunophenotyping, cytokine release, proteomics, or transcriptomics to read the DC and T-cell components behind the kill.
Reports you can act on
Progress reports let you adjust the plan as the study runs; final reports arrive with ready-to-publish data that holds up in due diligence.
Paul Marteau, PharmD (study director), Imane Nafia, PhD (CSO), Loïc Cerf, MSc (COO), Alban Bessede, PhD (founder, CEO), Jean-Philippe Guégan, PhD (CTO)
Contact our team
Discuss your antigen-specific killing study
Tell us your antigen or target, antigenic format, intended indication and therapeutic approach, and we'll scope the appropriate DC/CD8 model, tumor target, controls and functional readouts.
Frequently asked questions
Answers about antigen-specific killing
What does this assay measure?
The assay measures whether dendritic-cell-mediated antigen presentation generates a functional CD8 T-cell response capable of killing antigen-matched tumor cells. It can evaluate either the immunogenic potential of an antigenic candidate or the activity of a compound that modulates dendritic-cell function or T-cell priming.
How is this different from your other T-cell killing assays?
Our general T-cell–mediated tumor killing assays assess killing driven by immune activation, tumor sensitization or T-cell redirection. The antigen-specific assay adds an upstream dendritic-cell priming step to determine whether presentation of a defined antigen generates a functional tumor-reactive CD8 T-cell response.
What kinds of compounds can I test?
Antigenic candidates may include peptides, protein- or nucleic-acid-based vaccine constructs, tumor-derived materials and oncolytic viruses. The assay can also evaluate compounds that enhance dendritic cell maturation, antigen processing or presentation, or productive DC–CD8 T-cell interactions within a defined antigen–tumor system.
What are the readouts?
Core readouts are tumor-cell apoptosis and count by live-cell imaging (nuclear probe for count, caspase-3/7 reagent for death) and IFN-γ release as a functional readout of T-cell activation — IFN-γ in most models, with TNF-α, IL-6, or other cytokines added as the biology fits. On request: flow-cytometry immunophenotyping (including DC differentiation and maturation markers), broader cytokine panels, proteomics, and transcriptomics.
Which tumor models and antigens can you use?
The assay can be adapted to a wide range of tumor antigens and antigenic formats, including peptides, proteins, vaccine constructs, tumor-derived material and other sponsor-defined candidates. Tumor models are selected according to antigen expression, HLA compatibility and relevance to the intended indication. Autologous dendritic cells and CD8 T cells are then combined with the selected antigen-matched tumor target. A Melan-A / SK-MEL-5 system is available as a qualified reference model.
What controls establish antigen specificity?
Specificity is assessed by comparing CD8 T cells primed with antigen-exposed versus control dendritic cells and, where available, antigen-positive versus antigen-negative tumor targets. Additional controls may include CD8-only, tumor-only, and immune-cell-free conditions. Immune-modulating reference compounds can be included when relevant to the mechanism being studied.
Can you confirm the target antigen is expressed before the functional work?
Starting from FFPE cohorts, our translational team can explore or confirm target-antigen expression across cancer indications using single-cell RNA-seq, Xenium single-cell spatial transcriptomics, GeoMx digital spatial profiling, and digital pathology. See target expression profiling.
What do I receive?
A fit-for-purpose study design agreed up front, a dedicated PhD study director throughout, and a final report with the quantified killing and activation readouts, the requested mechanism add-ons, and a discussion of the results against your objectives.
