Immunohistochemistry & tissue-based analysis

In parallel with its flow cytometry-related know-how and platform, explicyte has extensive experience performing immunohistochemistry / immunohistofluorescence and related tissue analysis. Detection of immunohistochemical staining to assess specific markers characteristic either of cellular and molecular processes or to identify specific cell subsets as to profile an immune response to anti-cancer treatment, for instance, is an integral part of explicyte services that we are providing to build our clients’ studies and have an in-depth insight into the pattern characteristics and treatment responses. While we are continuously enriching our validated marker panels, our immunohistochemistry-related services are also widely proposed to study the expression, distribution and localization of intended biomarkers, in many types of tissues (tumors, xenografts, organs, normal tissue…) either frozen or paraffin-embedded, through either a standardized or customized work as to meet our specific clients’ needs.

Key advantages of tissue-based imaging

  • Exploration at multiple scales, from cell-to-cell interactions to the macroscopic tissue architecture.
  • Well-suited for simultaneous detection of multiple markers to identify specific cell subsets, document an immune response or quantify biomarkers of interest.
  • Automated and robust workflow from sample processing to multiplexed image acquisition and quantification.
  

Automated tissue slide imaging

Platform

Discovery XT (Ventana / Roche)

Staining type

  • Immunohistochemistry (IHC)
  • Immunohistofluorescence (IHF)

Sample format

  • Paraffin embedded tissues (FFPE sections)
  • Frozen tissues (Cryo-sections)
  • Tissue Micro Arrays (TMAs)
Multiplexed quantitative pathology imaging

Platform

Vectra Polaris (Perkin Elmer)

Sample format

Brightfield and fluorescence whole-slide scanning

Multispectral imaging

Up to 9 colors multiplexing

Readouts

Integrated tissue analysis software packages for  marker/biomarker quantification and spatial analysis

 


Illustrative data

Tumor infiltration in orthotopic glioma
Orthotopic glioma tumor-bearing model is characterized by tumor-infiltrating immune cells. Hematoxylin-associated IHC staining of brain sections of GL261 glioma-bearing mice (top left image) shows the presence of CD8+ cytotoxic lymphocytes infiltrating the tumor . Segmentation and quantification procedure of such CD8 staining is illustrated in the right images.

Orthotopic glioma tumor-bearing model is characterized by tumor-infiltrating immune cells. ...

Tumor infiltration in colon tumor
Tumor-infiltrating lymphocyte cells characterizing the subcutaneous syngeneic CT26 colon tumor model. Hematoxylin-associated IHC staining of CT26 tumors shows the presence of  cytotoxic CD8+ (A) and CD4+ (B) lymphocytes

Tumor-infiltrating lymphocyte cells characterizing the subcutaneous syngeneic CT26 colon tumor ...

Multispectral imaging
Illustration of multispectral imaging and color deconvolution process in brightfield imaging (IHC) that enables the identification and downstream quantification of multiple overlapping markers (CD8 in Red, CD163 in Blue, PDL1 in Purple and IDO1 in Brown) without the interference of autofluorescence as the signals are unmixed from one another.

Illustration of multispectral imaging and color deconvolution process in brightfield imaging ...